/

/

细胞外囊泡的量化和尺寸测定纳米粒子跟踪分析

JoVE Journal
Biologia

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Please note that all translations are automatically generated. Click here for the English version.

细胞外囊泡的量化和尺寸测定纳米粒子跟踪分析

DOI:

10.3791/62447-v

•

09:19 min

•

March 28, 2021

•

Nicole Comfort, Kunheng Cai, Tessa R. Bloomquist, Madeleine D. Strait, Anthony W. Ferrante Jr., Andrea A. Baccarelli

¹Department of Environmental Health Sciences,Columbia University Mailman School of Public Health, ²Department of Medicine,Naomi Berrie Diabetes Center, Columbia University

Capitoli

00:04Introduction
00:58Cuvette Preparation
01:53Particle Tracking Instrument Preparation and Nanoparticle Tracking Analysis
04:01NTA Data Processing, Display, and Interpretation
05:29Cuvette Cleaning
06:33Results: Using a Novel NTA Instrument to Estimate EV Size Distribution and Total Particle Concentration
08:33Conclusion

Summary

Traduzione automatica

我们演示了如何使用新型纳米粒子跟踪分析仪器来估计从小鼠围产层组织和人类血浆中分离出来的细胞外囊泡的大小分布和总颗粒浓度。

Tags

Nanoparticle Tracking Analysis Extracellular Vesicles NTA Parameters Sample Preparation Cuvette Particle Concentration Dilution Focus Frame Rate Exposure Time Stir Time Reproducibility Standardization

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

对于外来体定量和尺寸测量新方法研究

快速，特异微孔板检测为内和细胞外抗坏血酸在培养细胞的测定

对于外小泡的使用流式细胞仪分析技术

细胞调理介质细胞外囊泡的流式细胞学分析

卡诺尔哈布炎细胞外囊泡的纯化与分析

用于荧光标记大促卵体定量的自动成像和分析

通过共聚焦显微镜成像分析进行细胞外囊泡摄取测定

三维细胞外囊泡的直接随机光学重建显微镜

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

蓝藻细胞外囊泡的分离和表征

Read Article