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Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

DOI:
10.3791/62448-v

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12:38 min

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November 06, 2021

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, ,
1Department of Chemical and Materials Engineering, Donadeo Innovation Centre for Engineering,University of Alberta, 2School of Life Science and Engineering,Southwest Jiaotong University

Capitoli

  • 00:03Introduction
  • 02:08Design and Development of Peptide Library
  • 02:56In vitro Cell Culture
  • 04:42Fura-2 Calcium Assay
  • 05:35Cell Activation and Fluorescent Reading
  • 09:28Data Analysis
  • 09:38Representative Results
  • 12:13Conclusion

Summary

Traduzione automatica

Traduzione automatica

Techniques for generating a library of short peptides that can activate mast cells via the MRGPRX2 receptor are described. Associated techniques are easy, inexpensive, and can be extended to other cell receptors.

Tags

MRGPRX2Mast CellsPeptide LibraryAlanine ScanningN-TruncatedC-TruncatedN+C-TruncatedSolid Phase Peptide SynthesisFura-2 AM Calcium Sensitive DyeGrynkiewicz EquationCalcium Signaling

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