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¹H R_{1 +} RNA 放松分散实验样品制备和设置的实用方面

JoVE Journal
Biochimica

This content is Free Access.

JoVE Journal Biochimica

Practical Aspects of Sample Preparation and Setup of ¹H R_1ρ Relaxation Dispersion Experiments of RNA

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¹H R_{1 +} RNA 放松分散实验样品制备和设置的实用方面

DOI:

10.3791/62470-v

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08:17 min

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July 09, 2021

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Hannes Feyrer, Judith Schlagnitweit, Katja Petzold

¹Department of Medical Biochemistry and Biophysics,Karolinska Institutet

Capitoli

00:04Introduction
00:34NMR Sample Preparation
03:48¹H R_1ρ Relaxation Dispersion
05:29Results: Analysis of Produced Sample and Two Different Constructs Based on an RNA Hairpin
07:39Conclusion

Summary

Traduzione automatica

我们提出了一个协议，测量微到毫秒的动态¹³^C/15N标签和未标记RNA与^1H R₁₊放松分散核磁共振（NMR）光谱。此协议的重点在于高纯度样品制备和 NMR 实验的设置。

Tags

Keywords: RNA Sample Preparation NMR Setup Proton R1ρ Relaxation Dispersion Conformational Exchange In Vitro Transcription RNase H Cleavage HPLC Purification NMR Tube Cleaning D2O Addition

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