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Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
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JoVE Journal Ricerca sul cancro
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

DOI:
10.3791/62478-v

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05:49 min

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April 27, 2021

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, , , ,
1Department of Molecular Physiology and Biophysics, Carver College of Medicine,University of Iowa, 2Holden Comprehensive Cancer Center,University of Iowa, 3MD program, Carver College of Medicine,University of Iowa, 4Department of Pathology, Carver College of Medicine,University of Iowa, 5Department of Urology, Carver College of Medicine,University of Iowa, 6Department of Radiation Oncology, Carver College of Medicine,University of Iowa

Capitoli

  • 00:05Introduction
  • 00:39Cell Preparation
  • 01:41Fluid Shear Stress (FSS) Exposure
  • 02:49Viability Measurement
  • 03:59Results: Fluid Shear Stress Resistance of Mammary Epithelial Cancer Cells
  • 04:47Conclusion

Summary

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Here we demonstrate a method to apply fluid shear stress to cancer cells in suspension to model the effects of hemodynamic stress on circulating tumor cells.

Tags

Keywords: Hemodynamic StressCirculating Tumor CellsSyringeNeedleFluid Shear StressCancer CellsMetastatic CancerCancer Cell SuspensionCell ViabilityEnzymatic Assays

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