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小鼠巨核细胞祖细胞的分离
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Biologia
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JoVE Journal Biologia
Isolation of Mouse Megakaryocyte Progenitors
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小鼠巨核细胞祖细胞的分离

DOI:
10.3791/62498-v

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10:30 min

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May 20, 2021

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, , , ,
1Université de Strasbourg, INSERM UMR S1255, EFS Grand-EST, 2UMR-S1113 –IRFAC, Université de Strasbourg

Capitoli

  • 00:04Introduction
  • 00:38Mouse Bone Collection
  • 03:10Magnetic Depletion of Lineage Positive Cells
  • 07:17Results: Purification of MouseMEP and MKp from Femurs, Tibias, and Pelvic Bones by Flow Cytometry
  • 09:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

该方法描述了通过流式细胞术从小鼠股骨，胫骨和盆骨中纯化MEP和MKp。

Tags

MouseMegakaryocyteProgenitorsCell SortingMEPMKPBoneC57 Black SixIliac CrestFemurTibiaPBSNBCSDissectionCell Isolation

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