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将原发性癌症相关纤维细胞与乳腺癌的合成素 Murine 模型分离，用于靶向纳米粒子的研究

JoVE Journal
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JoVE Journal Ricerca sul cancro

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

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将原发性癌症相关纤维细胞与乳腺癌的合成素 Murine 模型分离，用于靶向纳米粒子的研究

DOI:

10.3791/62504-v

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08:02 min

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May 14, 2021

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Marta Truffi*¹, Leopoldo Sitia*², Marta Sevieri, Arianna Bonizzi, Maria Antonietta Rizzuto, Serena Mazzucchelli, Fabio Corsi²

¹Istituti Clinici Scientifici Maugeri IRCCS, ²Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”,Università di Milano, ³Dipartimento di Biotecnologie e Bioscienze,Università di Milano-Bicocca

Capitoli

00:04Introduction
00:37Tumor Dissociation into Single Cells
02:55Extraction of Cancer-Associated Fibroblasts (CAFs) from Dissociated Tumor
05:20Results-I: Isolation and Culture of Primary CAFs from a Syngeneic Murine Breast Cancer
06:40Results-II: Use of Isolated CAFs to Evaluate Targeting Potential of Hfn-FAP Functionalized Nanodrugs
07:08Conclusion

Summary

Traduzione automatica

本文旨在为三阴性乳腺癌的同步粘膜模型中原发性癌症相关成纤维细胞的分离与培养提供协议，并应用于旨在针对肿瘤微环境的新型纳米粒子的临床前研究。

Tags

Keywords: Primary Cancer-associated Fibroblasts Murine Breast Cancer Tumor Microenvironment In Vitro Studies Tumor Stroma Tumor Dissociation Cell Isolation Dead Cell Removal Magnetic Separation Cell Culture

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