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Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation
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Biologia
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JoVE Journal Biologia
Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation

Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation

DOI:
10.3791/62550-v

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05:25 min

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May 17, 2021

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, , , , ,
1Nano Flow Core Facility,Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Division of Allergy and Inflammation,Beth Israel Deaconess Medical Center

Capitoli

  • 00:04Introduction
  • 00:43Collection and Preparation of Blood for Positive Rhesus Factor Detection
  • 02:43Cell Analysis Using the Magnetic Levitation Device
  • 03:21Results: Analysis of the Separated Cells
  • 04:57Conclusion

Summary

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This paper describes a magnetic levitation-based method that can specifically detect the presence of antigens, either soluble or membrane-bound, by quantifying changes in the levitation height of capture beads with fixed densities.

Tags

Magnetic LevitationCell DensityAntigenic PropertiesMinimally InvasiveRhesus FactorFluorescent DyeDPBSHBSSGadoliniumIgG Control BeadsAnti-RhD Coated BeadsCapillary TubeCell SeparationCell DetectionAnemiaSepsis

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