Rivista
/
Biologia
/
Vurdering af global DNA-dobbeltstrenget endresektion ved hjælp af BrdU-DNA-mærkning kombineret med billeddannelse af cellecyklusdiskrimination
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Vurdering af global DNA-dobbeltstrenget endresektion ved hjælp af BrdU-DNA-mærkning kombineret med billeddannelse af cellecyklusdiskrimination

DOI:
10.3791/62553-v

•

06:44 min

•

April 28, 2021

•

, , ,
1Oncology Division,Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, 2Department of Molecular Biology, Medical Biochemistry, and Pathology,Laval University Cancer Research Center, 3Oncology Division,CHU de Québec Research Center, CHUL Pavilion

Capitoli

  • 00:05Introduction
  • 00:37Cell Culture, Treatments, and Coverslip Preparation
  • 01:18Pre-Extraction and Fixation
  • 02:13Permeabilization and Immunostaining
  • 03:53Image Analysis
  • 04:16Results: Visualization of DNA Double-Strand End Resection During S to G2 Phase of the Cell Cycle Using an Immunofluorescence-Based Method
  • 06:05Conclusion

Summary

Traduzione automatica

Traduzione automatica

I den nuværende protokol demonstrerer vi, hvordan man visualiserer DNA-dobbeltstrenget enderesektion under S / G2-fasen af cellecyklussen ved hjælp af en immunofluorescensbaseret metode.

Tags

DNA Double-strand BreakDNA ResectionCell CycleBrdU LabelingImmunostainingImaging

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Measuring Exocytosis in Neurons Using FM Labeling

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Måling cellecyklusprogression Kinetics med metabolisk mærkning og Flowcytometri

Levende cellecyklusanalyse af<em> Drosophila</em> Væv bruger Attune Acoustic Fokusering Cytometer og Vybrant DyeCycle Violet DNA Stain

Real-time Imaging af Single Engineered RNA-transkripter i levende celler Brug Ratiometrisk bimolekylær Beacons

Diskrimination og karakterisering af heterocellulære populationer ved anvendelse af kvantitative billedteknikker

3' enden sekventering bibliotek forberedelse med A-seq2

Read Article