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Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes
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JoVE Journal Genetica
Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes

Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes

DOI:
10.3791/62588-v

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07:45 min

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May 01, 2021

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, , , ,
1Department of Microbiology & Molecular Genetics,University of California, Irvine, 2Department of Molecular Biology & Biochemistry,University of California, Irvine, 3Vector Biology Department,Liverpool School of Tropical Medicine

Capitoli

  • 00:04Introduction
  • 00:49Inundative Release Trials on Non-Gene Drive Mosquitoes
  • 02:59Overlapping Generation Trials of Gene-Drive Mosquitoes
  • 04:17Non-overlapping Generation Trials of Gene-Drive Mosquitoes
  • 05:59Results: Predicted Transgene Fixation Dynamics Over Seven Generations
  • 07:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

The protocols reported here illustrate three alternative ways to assess the performance of genetically-engineered mosquitoes destined for vector control in laboratory-contained small cage trials. Each protocol is tailored to the specific modification the mosquito strain bears (gene drive or non-gene drive) and the types of parameters measured.

Tags

Genetically-engineered Anopheline MosquitoesSmall-cage Laboratory TrialsTransgenic Mosquito PerformanceMalaria EliminationTrans-gene DynamicsWild Type LarvaeBlood MealOvipositionLarval ScreeningGene Drive ExperimentBarrier StrategyMale Release Ratio

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