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Microinjection Method for Anopheles gambiae Embryos
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JoVE Journal Genetica
Microinjection Method for Anopheles gambiae Embryos

Microinjection Method for Anopheles gambiae Embryos

DOI:
10.3791/62591-v

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05:39 min

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July 07, 2021

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, , ,
1Department of Microbiology & Molecular Genetics,University of California, Irvine, 2Department of Molecular Biology & Biochemistry,University of California, Irvine

Capitoli

  • 00:05Introduction
  • 00:51Embryo Preparation
  • 01:53Embryo Alignment
  • 02:35Embryo Microinjection
  • 03:53Results: Microinjection Method to Target Agcd Gene Ortholog Using pCO37 Gene Drive Construct
  • 05:11Conclusion

Summary

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Microinjection techniques are essential to introduce exogenous genes into the genomes of mosquitoes. This protocol explains a method used by the James laboratory to microinject DNA constructs into Anopheles gambiae embryos to generate transformed mosquitoes.

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Keywords: MicroinjectionAnopheles GambiaeEmbryosTransgenicMalariaPopulation SuppressionMosquito SpeciesAspiratorConical TubeDental FilmNylon MeshFilter PaperIncubatorForcepsPetri DishMembraneFilter PaperMicroloader TipNeedle HolderAutomated Pressure Pump

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