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Using Baseplating and a Miniscope Preanchored with an Objective Lens for Calcium Transient Research in Mice
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Capitoli
- 00:05Introduction
- 00:42Adeno-Associated Virus (AAV) Injection and Relay Lens Implantation
- 01:45Dummy Baseplating
- 02:44Baseplating
- 04:39Results: Assessment of Dental Cement and Monitoring Baseplating Procedure
- 05:45Conclusion
The shrinkage of dental cement during curing displaces the baseplate. This protocol minimizes the problem by creating an initial foundation of the dental cement that leaves space to cement the baseplate. Weeks later, the baseplate can be cemented in position on this scaffold using little new cement, therebyreducing shrinkage.
Tags
BaseplatingMiniscopePreanchoredObjective LensCalcium TransientMiceFluorescence SignalsViral VectorRelay LensDental CementStereotaxic ArmMicroinjectionVentral Cornu Ammonis 1Pyrogen-free SalineMicro Bulldog ClampHeat Shrink TubingParaffin FilmReusable Adhesive ClayMolding Silicone RubberBone Rongeur75% AlcoholSet ScrewFocus Slide
