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Journal / Genetics / 使用废培养基对人类植入前胚胎进行染色体筛选：样品采集和染色体倍性分析…
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JoVE Journal Genetics
使用废培养基对人类植入前胚胎进行染色体筛选：样品采集和染色体倍性分析
 
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使用废培养基对人类植入前胚胎进行染色体筛选：样品采集和染色体倍性分析

Article DOI: 10.3791/62619-v • 12:32 min • September 7th, 2021
September 7th, 2021 •
*1,2,3,4, *5, 1,2,3,4, 1,2,3,4, 5, 5, 1,2,3,4, 5
1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education, Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research, Yikon Genomics Co. Ltd.
* These authors contributed equally

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本研究报告了一种使用废培养基对人类胚胎进行染色体筛选的方案，该方案避免了胚胎活检并使用 NGS 进行染色体倍性鉴定。本文介绍了详细的过程，包括培养基的制备、全基因组扩增（WGA）、下一代测序（NGS）文库的制备和数据分析。

Tags

染色体筛查、人类胚胎植入前胚胎、废培养基、样本采集、染色体倍性分析、体外受精 （IVF）、非整倍体植入前基因检测 （PGT-A）、滋养外胚层 （TE）、细胞内质量 （ICM）、假阳性和假阴性、NICS 技术、DNA 测序、培养基采样、全基因组扩增 （WGA）、文库制备、NGS 数据分析、冷冻保存、试管婴儿实验室
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