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JoVE Journal Cancer Research
Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product
 

Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

Article DOI: 10.3791/62622-v • 11:51 min • September 22nd, 2021
September 22nd, 2021 •
1, 1, 2, 1,2, 1,2, 1,2
1Cell Therapy Facility, Moffitt Cancer Center and Research Institute, 2Department of Immunology, Moffitt Cancer Center and Research Institute

Capitoli

Riepilogo
Automatic Translation

Presented is a protocol for the expansion of Gamma-Delta (γδ) T cell drug product. Lymphocytes are isolated by elutriation and γδ-enriched with zoledronic acid and interleukin-2. Alpha-beta T cells are depleted using a clinical-grade magnetic separation device. The γδ cells are co-cultured with K562-derived, artificial antigen-presenting cells and expanded.

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Keywords: Gamma-delta T Cells Cytotoxic Activity Cancer Acute Myeloid Leukemia Expansion Enrichment Apheresis Cell Therapy Elutriation Cell Culture Bioreactor Zoledronic Acid IL-2 Flow Cytometry
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