Rivista
/
Medicina
/
使用GCaMP3报告器测量心脏特异性钙通量，对心脏重编程进行体外评估
JoVE Journal
Medicina
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Medicina
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
Please note that all translations are automatically generated. Click here for the English version.

使用GCaMP3报告器测量心脏特异性钙通量，对心脏重编程进行体外评估

DOI:
10.3791/62643-v

•

05:04 min

•

February 22, 2022

•

, ,
1Department of Cardiac Surgery, Cardiovascular Center,The University of Michigan, Ann Arbor, 2Department of Cardiovascular Medicine, Xiangya Hospital,Central South University

Capitoli

  • 00:04Introduction
  • 00:53Isolation and Selection of Neonatal Myh6-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 Mice Hearts
  • 01:23Isolation of Neonatal Cardiac Fibroblasts (NCFs)
  • 02:43Reprogramming NCFs to iCMs with MGT Encoding Retrovirus Infection
  • 03:28Results: In Vitro Assessment of Cardiac Reprogramming
  • 04:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们在这里描述了Tg（Myh6-cre）1Jmk/J /Gt（ROSA）26Sortm38（CAG-GCaMP3）Hze/J（下面称为αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3）小鼠报告线用于心脏重编程评估的建立和应用。从小鼠菌株中分离出的新生儿心脏成纤维细胞（NCF）转化为诱导心肌细胞（iCM），从而可以方便有效地评估通过钙（Ca2 +）通量对iCM的重编程效率和功能成熟。

Tags

Keywords: Cardiac ReprogrammingCalcium FluxGCaMP3 ReporterNeonatal Cardiac FibroblastsIsolationCell CultureFunctional MaturationCardiomyocyte DifferentiationCardiac Function

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/62643?article_type=v

多巴酚丁胺诱导的心脏压力测试和监控通过在小鼠心脏导管插入术
check_url/it/62643?article_type=v

测量心脏自主神经系统（ANS）儿童活动
check_url/it/62643?article_type=v

直接压力监测准确预测在Cryoballoon消融肺静脉阻塞
check_url/it/62643?article_type=v

右心室结构和功能的肺动脉缩窄经胸超声小鼠模型的评估
check_url/it/62643?article_type=v

心脏异常的实验性自身免疫性心肌炎的无创性评估磁共振成像显微镜在鼠标
check_url/it/62643?article_type=v

诱导和缺血再灌注损伤评估中的Langendorff灌注鼠心
check_url/it/62643?article_type=v

在横向主动脉缩窄的小鼠模型和心脏形态学评估功能改变通过超声心动图成像
check_url/it/62643?article_type=v

制备和<em>体外</em>磁化的表征的miR-改性内皮细胞
check_url/it/62643?article_type=v

成人大鼠心脏解剖与功能的超声心动图评估
check_url/it/62643?article_type=v

使用皮肤心肌细胞进行心脏功能的体外评估

Read Article