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Retrograde Parotid Gland Infusion through Stensen’s Duct in a Non-Human Primate for Vectored Gene Delivery
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Retrograde Parotid Gland Infusion through Stensen’s Duct in a Non-Human Primate for Vectored Gene Delivery

Retrograde Parotid Gland Infusion through Stensen’s Duct in a Non-Human Primate for Vectored Gene Delivery

DOI:
10.3791/62645-v

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03:50 min

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August 12, 2021

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, , , , ,
1Department of Medicine, Division of Infectious Diseases and Global Medicine,University of Florida, 2Department of Otolaryngology,George Washington University School of Medicine, 3Department of Medicine, Section on Infectious Diseases,Wake Forest Baptist Medical Center, 4Department of Pathology,Wake Forest School of Medicine, 5Department of Medicine, Section of Infectious Diseases,Hefner Veterans Affairs Medical Center

Capitoli

  • 00:04Introduction
  • 00:49Performing the Procedure
  • 02:49Results: Successful Transduction Following Retrograde Parotid Gland Infusion of Ad5-EGFP in a Non-Human Primate
  • 03:22Conclusion

Summary

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Salivary glands have been proposed as a tissue target site for gene therapy, especially in the area of vaccination by gene transfer. We demonstrate gene delivery in a non-human primate model utilizing retrograde parotid infusion.

Tags

Retrograde Parotid Gland InfusionStensen’s DuctNon-human PrimateVectored Gene DeliveryParotid PapillaPolyethylene TubeCyanoacrylateFluoroscopyEGFPGene TherapySalivary Gland

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