Rivista
/
Ricerca sul cancro
/
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
JoVE Journal
Ricerca sul cancro
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ricerca sul cancro
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

DOI:
10.3791/62651-v

•

09:19 min

•

July 06, 2022

•

, , , , ,
1Department of Pathology,Genentech, 2Department of Microchemistry, Proteomics, Lipidomics & Next Generation Sequencing (MPL-NGS),Genentech, 3NanoString Technologies

Capitoli

  • 00:05Introduction
  • 00:44Automated Visualization Protocol for Spatial Proteomics Assays
  • 06:02Spatial Profiling Platform Protocol for Proteomics Assay
  • 06:47ROI Selection for Spatial Transcriptomics Assays
  • 07:10Results: Application of the Automated TSA Based Visualization Protocol in Combination with Spatial Proteomics Assays and Detection of Small ROIs in the Spatial Transcriptomics Protocol
  • 08:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we describe a protocol for fine-tuning regions of interest (ROIs) for Spatial Omics technologies to better characterize the tumor microenvironment and identify specific cell populations. For proteomics assays, automated customized protocols can guide ROI selection, while transcriptomics assays can be fine-tuned utilizing ROIs as small as 50 µm.

Tags

Spatial ProfilingProtein ExpressionRNA ExpressionVirtual MicrodissectionProteomicsTranscriptomicsAutomated ProtocolRegion Of Interest (ROI) SelectionFluorescent Visualization AntibodiesAutostainerDeparaffinizationCell ConditioningAntibody IncubationHRP BlockerMultimer HRP ReagentAntibody Denaturation

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

In Vitro Assay to Study Tumor-macrophage Interaction

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2

Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging

Read Article