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Immunologia e infezioni

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从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

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从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法

DOI:

10.3791/62654-v

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08:05 min

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May 06, 2021

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Qi Zhou*^1,2,3, Xiaoling Xie*^1,2,3, Zhiqian Zhong^2,3, Pingnan Sun^2,3, Xiaoling Zhou^2,3

¹Stem Cell Research Center,Shantou University Medical College, ²The Center for Reproductive Medicine,Shantou University Medical College, ³Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Capitoli

00:04Introduction
00:45Stem Cell Maintenance
02:28Stem Cell Passage and Differentiation and Definitive Endoderm Formation
03:46Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
05:12Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
07:14Conclusion

Summary

Traduzione automatica

本手稿描述了人类胚胎干细胞（hESC）分化成功能性肝细胞样细胞（HLCs）的详细协议，在hESC分化过程中连续补充Activin A和CHIR99021，以最终内分泌体（DE）。

Tags

Keywords: Human Embryonic Stem Cells Hepatocyte-like Cells Differentiation Definitive Endoderm FOXA2 SOX17 GATA4 CXCR4 FOXA1 Matrigel MTESR Activin A CHIR99021 Cell Culture Disease Modeling Drug Screening

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