Rivista
/
/
Flowcytometrisk analyse av apoptotiske biomarkører i Actinomycin D-behandlede SiHa livmorhalskreftceller
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
DOI:

13:53 min

August 26, 2021

, ,

Capitoli

  • 00:04Introduction
  • 00:43Preparation of Cell Culture and Treatments for Flow Cytometry
  • 02:56Enumerating Cells Using the Viability Assay
  • 03:36Staining Cells for Flow Cytometry
  • 08:00Run Samples on the Flow Cytometer
  • 10:04Post-Acquisition and Statistical Analysis
  • 11:20Results: Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
  • 13:01Conclusion

Summary

Traduzione automatica

Apoptose kan karakteriseres ved flowcytometrisk analyse av tidlige og sene apoptotiske biomarkører. Cellelinjen for livmorhalskreft, SiHa, ble analysert for apoptosebiomarkører etter behandling med Actinomycin D ved hjelp av et benchtop flow cytometer.

Video correlati

Read Article