Rivista
/
Biologia dello sviluppo
/
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
JoVE Journal
Biologia dello sviluppo
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

DOI:
10.3791/62667-v

•

14:19 min

•

March 25, 2022

•

, , , , , , , , , , , , , , , ,
1Imagine Institute, INSERM UMR 1163,Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163,Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163,Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades,Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology,APHP- Necker Enfants Malades Hospital

Capitoli

  • 00:07Introduction
  • 01:42Generation of 2D hIPS Cell-Based Models of Neocortical Development
  • 02:56Generation of Dorsal Forebrain Organoids
  • 04:56In toto Immunolabeling, Clearing, and Lightsheet Acquisition of Dorsal Forebrain Organoids
  • 07:00Immunostaining and Clearing of Free-Floating Sections of Dorsal Forebrain Organoids
  • 09:20Representative Results
  • 13:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present detailed protocols for the generation and characterization of 2D and 3D human induced pluripotent stem cell (hIPSC)-based models of neocortical development as well as complementary methodologies enabling qualitative and quantitative analysis of primary cilium (PC) biogenesis and function.

Tags

2D3DHumanInduced Pluripotent Stem CellPrimary CiliumNeocortical DevelopmentNeural RosettesDorsal Forebrain OrganoidsEmbryoid BodyDual SMAD InhibitionImmunohistochemistryVibratomeConfocal Microscopy

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/62667?article_type=v

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
check_url/it/62667?article_type=v

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
check_url/it/62667?article_type=v

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
check_url/it/62667?article_type=v

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
check_url/it/62667?article_type=v

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/it/62667?article_type=v

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
check_url/it/62667?article_type=v

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
check_url/it/62667?article_type=v

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
check_url/it/62667?article_type=v

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
check_url/it/62667?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Read Article