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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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Capitoli
- 00:04Introduction
- 00:31Resin-Assisted Capture
- 03:52On-Resin Tryptic Digestion and Tandem Mass Tag (TMT) Labeling
- 04:45Peptide Elution
- 05:47Results: Analysis of Peptides from Resin-Assisted Capture Enrichment
- 06:35Conclusion
Protein thiol oxidation has significant implications under normal physiological and pathophysiological conditions. We describe the details of a quantitative redox proteomics method, which utilizes resin-assisted capture, isobaric labeling, and mass spectrometry, enabling site-specific identification and quantification of reversibly oxidized cysteine residues of proteins.
