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基于毛细管电泳的氢/氘交换，用于自上而下的质谱法对蛋白质进行构象表征

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Chimica

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JoVE Journal Chimica

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

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基于毛细管电泳的氢/氘交换，用于自上而下的质谱法对蛋白质进行构象表征

DOI:

10.3791/62672-v

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05:45 min

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June 08, 2021

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Lingxiao Chaihu², Xiaopeng Yao, Xiang Xu², Zhongqin Zhu, David D. Y. Chen, Guanbo Wang

¹School of Chemistry and Materials Science,Nanjing Normal University, ²Institute for Cell Analysis,Shenzhen Bay Laboratory, ³Department of Chemistry,University of British Columbia

Capitoli

00:04Introduction
00:38Operation of CE-Based HDX MS Analysis
04:01Results: Conformational Characterization of Proteins with Top-Down Mass Spectrometry
05:18Conclusion

Summary

Traduzione automatica

这里介绍的是基于毛细管电泳的氢/氘交换（HDX）方法与自上而下的质谱法相结合的方案。该方法通过同时进行差分HDX和电泳分离来表征不同蛋白质物种（包括不同状态的蛋白质和不同蛋白质形式）之间高阶结构的差异。

Tags

Capillary Electrophoresis Hydrogen/deuterium Exchange Conformational Characterization Top-down Mass Spectrometry Protein Species Separation Higher-order Structure HDX-CE-MS Electrophoretic Effect Preconditioning Ultrasonication Background Electrolyte Infusion Tubing Nano-electrospray Ionization Injection Volume Estimation Electrophoretic Voltage

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