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确定紫外线辐射和化学物质对原代和永生化的人角膜上皮细胞的毒性

JoVE Journal
Biologia

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JoVE Journal Biologia

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

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确定紫外线辐射和化学物质对原代和永生化的人角膜上皮细胞的毒性

DOI:

10.3791/62675-v

•

09:31 min

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July 22, 2021

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Jaclyn M. L. Chang, Junghee Seo, Maggie Miu Yee Kwan, Sarah Oh, David J. McCanna, Lakshman Subbaraman, Lyndon Jones²

¹Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science,University of Waterloo, ²Centre for Eye and Vision Research (CEVR)

Capitoli

00:05Introduction
00:56Determination of Cell Size Using Confocal Microscopy
01:59Exposure of Cells to Ultraviolet (UV) Radiation
03:50Exposure of Cells to Chemical Toxins
05:31Examination of Cells Exposed to Various Concentrations of BAK
06:53Results: Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
08:50Conclusion

Summary

Traduzione automatica

本文介绍了用于评估紫外线辐射和化学毒素对原代和永生化细胞系的毒性的程序。

Tags

Keywords: Toxicity UV Radiation Chemicals Primary Human Corneal Epithelial Cells Immortalized Human Corneal Epithelial Cells In Vitro Assay Cell Viability Annexin Staining Confocal Microscopy Cytokine Release

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