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秀丽隐杆线虫树突棘成像
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Imaging Dendritic Spines in Caenorhabditis elegans
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秀丽隐杆线虫树突棘成像

DOI:
10.3791/62676-v

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09:14 min

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September 27, 2021

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1Department of Cell and Developmental Biology,Vanderbilt University, 2Program of Neuroscience,Vanderbilt University

Capitoli

  • 00:05Introduction
  • 00:53Determination of Dorsal D (DD) Dendritic Spines Structure
  • 02:44Assessing Activation of DD Dendritic Spines by Presynaptic Cholinergic Signaling
  • 07:06Results: Imaging and Assessing the Function of DD Dendritic Spines in Caenorhabditis elegans
  • 08:37Conclusion

Summary

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树突棘是神经系统的重要细胞特征。这里描述了用于评估 秀丽隐杆线虫树突棘结构和功能的实时成像方法。这些方法支持开发定义树突棘形状或功能的基因的突变筛选。

Tags

Keywords: Dendritic SpinesCaenorhabditis ElegansGABA NeuronsImmobilizationSuper-resolution MicroscopyNyquist Acquisition3D-deconvolutionSpine DensitySpine ClassificationCalcium Sensor GCaMPChrimsonAll-trans-retinal

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