È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Introduction
- 00:30Imaging Medium Preparation
- 00:48Observation-Chamber Preparation
- 01:13Ovary Dissection
- 01:51Egg Chamber Isolation
- 02:29Observation Chamber Closing
- 02:56Results: Nuclear Migration
- 03:41Conclusion
In Drosophila, the oocyte nucleus undergoes microtubule-dependent migration during oogenesis. Here, we describe a protocol that was developed to follow the migration by performing live imaging on egg chambers ex-vivo. Our procedure maintains egg chambers alive for 12 h to acquire multi-position 3D time-lapse movies using spinning-disk confocal microscopy.
