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1Institute of Pharmacology and Toxicology,University Medical Center Goettingen, 2DZHK (German Center for Cardiovascular Research) partner site, Goettingen, 3Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC),University of Goettingen, 4Center for Neurodegenerative Diseases (DZNE), 5Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP)
Capitoli
- 00:04Introduction
- 00:52Primary Cardiac Fibroblast Preparation
- 01:39Enzymatic Dispersion of Human Cardiac Fibroblasts
- 02:52Engineered Connective Tissue Preparation
- 05:28Cross-Sectional Area Measurement to Assess ECT Compaction
- 05:55Pole Deflection Analysis to Monitor ECT Contraction
- 06:32Destructive Tensile Measurement and Stress-Strain Analysis to Assess ECT
- 08:20Results: Effect of Actin Polymerization on ECT Compaction, Contraction, and Stiffness
- 09:22Conclusion
Presentert her er en protokoll for å generere konstruert bindevev for en parallell kultur på 48 vev i en multi-brønn plate med doble poler, egnet for mekanistiske studier, sykdomsmodellering og screening applikasjoner. Protokollen er kompatibel med fibroblaster fra forskjellige organer og arter og eksemplifiseres her med humane primære hjertefibroblaster.
