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Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
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Neuroscienze
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JoVE Journal Neuroscienze
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

DOI:
10.3791/62717-v

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08:31 min

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July 29, 2021

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, , , , ,
1The Brain Institute at Florida Atlantic University, 2Department of Biomedical Sciences,Florida Atlantic University, 3Department of Chemistry and Biochemistry,Florida Atlantic University

Capitoli

  • 00:00Introduction
  • 01:13Microscope Setup, Loading and Imaging ND6 in Neuronal Cultures
  • 04:17Quantification of Membrane Trafficking by a Change in ND6 Fluorescence
  • 06:44Results: Membrane Lipid Turnover with the pH‐Sensitive Fluorescent Lipid Analog ND6
  • 08:00Conclusion

Summary

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This protocol presents a fluorescence imaging method that uses a class of pH-sensitive lipid fluorophores to monitor lipid membrane trafficking during cell exocytosis and the endocytosis cycle.

Tags

Membrane Lipid TurnoverPH-sensitive Fluorescent Lipid AnalogND6ExocytosisEndocytosisLive Cell Fluorescence ImagingCell MembraneMembrane TraffickingLipid MimeticPiperazine Head GroupFluorescence IntensityProtonationDeprotonationCell BiologyBiomedical Sciences

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