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Immunology and Infection

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JoVE Journal Immunology and Infection
Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
 

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Article DOI: 10.3791/62729-v • 09:10 min • July 16th, 2021
July 16th, 2021 •
1, 1, 1, 1, 1, 1
1Molloy College

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Presented here is a protocol to quantify and produce dynamic images of mesenchymal stem cell (MSC) mediated regulation of macrophage (MΦ) phagocytosis of non-opsonized yeast (zymosan) particles that are conjugated to a pH-sensitive fluorescent molecule.

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Keywords: Mesenchymal Stem Cells Macrophage Phagocytosis Cell Culture Co-culture Bioparticles PH-sensitive Dyes Phagocytes Cell Counting Trypan Blue Hemocytometer Cell Suspension
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