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使用双氟比度显微镜测量巨无素体的pH值、重度化学和降解能力

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

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使用双氟比度显微镜测量巨无素体的pH值、重度化学和降解能力

DOI:

10.3791/62733-v

•

07:31 min

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August 19, 2021

•

Liam Wilkinson², Johnathan Canton³

¹Department of Biochemistry & Microbiology, Faculty of Science,University of Victoria, ²Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Science,University of Calgary, ³Calvin, Joan and Phoebe Snyder Institute for Chronic Diseases,University of Calgary

Capitoli

00:05Introduction
00:28Measuring Macropinosome pH
02:06In Situ Calibration of Macropinosome pH
03:04Measuring Oxidative Events within Macropinosomes
04:22Measuring Protein Digestion within Macropinosomes
05:30Results: Measuring the pH, Oxidative Events, and Protein Degradation in Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
06:59Conclusion

Summary

Traduzione automatica

我们描述了测量活细胞中单个巨细胞体中的pH值、氧化事件和蛋白质消化的方案。强调双氟比显微镜及其提供的优势，而不是基于人群的技术。

Tags

Macropinosomes PH Redox Degradation Dual-fluorophore Ratiometric Microscopy Fluorescein TMR HBSS Potassium Rich Solution Nigericin RAW264.7 Cells

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