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Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate
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Biologia
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JoVE Journal Biologia
Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate

Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate

DOI:
10.3791/62753-v

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06:03 min

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October 29, 2021

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, , ,
1Biocircuits Institute,University of California, San Diego, 2Present address,Kyoto Institute of Technology, 3Present address,Vertex Pharmaceuticals, 4Department of Bioengineering,University of California, San Diego, 5Molecular Biology Section,University of California, San Diego

Capitoli

  • 00:04Introduction
  • 00:38Cell Preparation
  • 02:05Lysate Preparation
  • 04:13Cell-Free Gene Expression
  • 04:47Results: Green Fluorescent Protein (GFP) Expression Using the Autolysate
  • 05:18Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes a rapid and simple method to produce bacterial lysate for cell-free gene expression, using an engineered strain of Escherichia coli and requiring only standard laboratory equipment.

Tags

Bacterial Cell-free LysateCell-free Gene ExpressionAutolysis E. ColiLB AgarAmpicillinStarter Culture2xYTPG MediumOptical DensityCentrifugationS30A BufferDithiothreitol

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