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Generation of Human Brain Organoids for Mitochondrial Disease Modeling
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JoVE Journal Neuroscienze
Generation of Human Brain Organoids for Mitochondrial Disease Modeling

Generation of Human Brain Organoids for Mitochondrial Disease Modeling

DOI:
10.3791/62756-v

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08:09 min

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June 21, 2021

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, , , , , , , ,
1Department of General Pediatrics, Neonatology and Pediatric Cardiology, Duesseldorf University Hospital, Medical Faculty,Heinrich Heine University, 2Institute of Neurobiology,Heinrich Heine University, 3Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrueck Center for Molecular Medicine (MDC), 4Seaver Autism Center for Research and Treatment,Icahn School of Medicine at Mount Sinai, 5Max Delbrueck Center for Molecular Medicine (MDC)

Capitoli

  • 00:05Introduction
  • 00:44Generation of Brain Organoids
  • 05:31Immunostaining of Brain Organoids
  • 06:07Results: Visualization and Bioenergetic Profiling of Brain Organoids Generated from iPSCs
  • 07:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a detailed protocol for the generation of human induced pluripotent stem cell-derived brain organoids and their use in modeling mitochondrial diseases.

Tags

Brain OrganoidsMitochondrial DiseasesIPSCsCortical DifferentiationNeurospheresROCK InhibitorWNT InhibitorSB-431542Cell CultureReproducibility

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