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Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings

Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings

DOI:
10.3791/62760-v

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07:37 min

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August 05, 2021

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, , , , ,
1Department of Biology,Washington University in St. Louis, 2Department of Biology, Program in Neuroscience,Brandeis University

Capitoli

  • 00:05Introduction
  • 00:42Preparation of Carbon Fibers and Tools
  • 01:25Assembling the Carbon Fiber Microelectrode Array
  • 04:20Electrode-tip Preparation
  • 05:44Results: Construction and Implementation of Carbon Fiber Microelectrode Arrays for Recording Neural Activity
  • 07:09Conclusion

Summary

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This protocol describes a procedure for constructing carbon fiber microelectrode arrays for chronic and acute in vivo electrophysiological recordings in mouse (Mus musculus) and ferret (Mustela putorius furo) from multiple brain regions. Each step, following the purchase of raw carbon fibers to microelectrode array implantation, is described in detail, with emphasis on microelectrode array construction.

Tags

Carbon FiberMicroelectrode ArrayIn Vivo RecordingsChronic RecordingsAcute RecordingsNeuron RecordingsElectrode ConstructionBiocompatibleBrain-computer InterfaceNeural Interface

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