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인간 종양 세포 및 종양-xenografted 마우스에 있는 매크로 피노세포증에 의해 중재된 ATP 내재화를 위한 형광 현미경 검사법
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1Department of Biological Sciences,Ohio University, 2Molecular and Cellular Biology Program,Ohio University, 3Neuroscience Program,Ohio University, 4Edison Biotechnology Institute,Ohio University, 5Translational Biomedical Sciences Program,Ohio University, 6Honors Tutorial College,Ohio University, 7Department of Biomedical Sciences, Heritage College of Osteopathic Medicine,Ohio University
Capitoli
- 00:05Introduction
- 01:05Tumor Cell Culture and Xenograft Tumor Development
- 02:41HMWFD and NHF-ATP Solution Preparation
- 03:21ATP Internalization in Tumor Xenograft
- 03:53Cryo-embedding
- 04:45Tissue Slide Fixation
- 05:25Fluorescence Microscopy and Image Acquisition
- 07:12Results: Assessment of Nonhydrolyzable Fluorescent ATP Internalization
- 07:44Conclusion
