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基于时空波动光谱的运输亚细胞纳米结构的结构和动态性质
JoVE Journal
Biologia
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JoVE Journal Biologia
Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
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基于时空波动光谱的运输亚细胞纳米结构的结构和动态性质

DOI:
10.3791/62790-v

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08:17 min

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August 16, 2021

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, , ,
1NEST Laboratory,Scuola Normale Superiore, 2Center for Nanotechnology Innovation@NEST CNI@NEST

Capitoli

  • 00:04Introduction
  • 00:58Sample Preparation
  • 02:35Data Acquisition
  • 03:52iMSD Calculation
  • 05:54Results: Structural and Dynamic Properties of Lysosomes and Macropinosomes
  • 07:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

成像衍生的均方位移（iMSD）分析应用于大刺激小体，以突出它们在结构和动态特性方面的内在时间演化性质。然后将大孔蛋白酶体与胰岛素分泌颗粒（ISG）进行比较，作为具有时间不变平均结构/动态特性的亚细胞结构的参考。

Tags

Keywords: Trafficking Subcellular NanostructuresSpatiotemporal Fluctuation SpectroscopyImaging The Right Mean Square DisplacementSubcellular Nano-structuresCell LabelingTime-lapsed Series AcquisitionFluorescence ImagingEGFPMacropinesomes

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