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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

DOI:
10.3791/62799-v

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12:54 min

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October 02, 2021

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, , , , , ,
1Auckland Bioengineering Institute,University of Auckland, 2Department of Engineering Science,University of Auckland

Capitoli

  • 00:00Introduction
  • 01:26Isolate a Trabecula
  • 03:18Prepare the Cardiomyometer
  • 04:40Mount the Trabecula
  • 05:57Prepare the Trabecula
  • 07:55Collect Brightfield and Fluorescence Imaging Data
  • 08:53Collect Oct Imaging Data
  • 09:55Representative Results
  • 12:26Conclusions

Summary

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This protocol presents a collection of sarcomere, calcium, and macroscopic geometric data from an actively contracting cardiac trabecula ex vivo. These simultaneous measurements are made possible by the integration of three imaging modalities.

Tags

Simultaneous ImagingBrightfieldFluorescenceOptical Coherence TomographyCardiac TrabeculaeMechanical FunctionIonic DynamicsGeometrical ChangeHeterogeneityMuscle ActivityEx VivoHeart DiseaseTreatment StrategiesDichroic MirrorsFluorescence EmissionBrightfield TransmissionIndependently-actuated HooksMeasurement ChamberExternal TriggerAnesthetized RatPerfusion CannulaCoronary VasculatureSeptumFree-running TrabeculaSpring Scissors

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