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Automatiseret mikrobiel dyrkning og adaptiv evolution ved hjælp af mikrobielt mikrodrøblekultursystem (MMC)
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1Department of Chemical Engineering, Institute of Biochemical Engineering,Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education,Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology,Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology,Tsinghua University, 6School of Life Science and Technology,Tokyo Institute of Technology
Capitoli
- 00:04Introduction
- 00:51Preparations for the Experiment
- 01:48Growth Curve Measurement in Microbial Microdroplet Culture System (MMC)
- 04:25Adaptive Evolution in Microbial Microdroplet Culture System (MMC)
- 06:41Results: The Adaptive Evolution of the Methanol-Essential Escherichia coli (MeSV2.2) in MMC
- 07:36Conclusion
Denne protokol beskriver, hvordan man bruger det mikrobielle mikrodropletkultursystem (MMC) til at udføre automatiseret mikrobiel dyrkning og adaptiv evolution. MMC kan dyrke og underdyrke mikroorganismer automatisk og kontinuerligt og overvåge deres vækst online med relativt høj gennemstrømning og god parallelisering, hvilket reducerer arbejdskraft- og reagensforbruget.
Tags
Automated Microbial CultivationAdaptive EvolutionMicrobial Microdroplet Culture System (MMC)Low-costOperation-friendlyReliable ResultsSimple OperationsMicrobiology ResearchGrowth Curve MeasurementAdaptive Laboratory EvolutionSingle Factor Multi-level AnalysisMicrofluidic ChipEscherichia Coli MG1655Cultivation TemperaturePhotoelectric SignalMMC OilInitial Bacteria Solution
