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肌萎缩性侧索硬化症模型中突触功能的实时荧光测量
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Neuroscienze
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JoVE Journal Neuroscienze
Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis
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肌萎缩性侧索硬化症模型中突触功能的实时荧光测量

DOI:
10.3791/62813-v

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08:59 min

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July 16, 2021

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, , ,
1Jefferson Weinberg ALS Center,Thomas Jefferson University

Capitoli

  • 00:04Introduction
  • 00:52Microscope and Perfusion System Setup
  • 02:33Styryl Dye Imaging of Synaptic Vesicle Release
  • 04:30Fluorescence Imaging of Gcamp6m Calcium Transients
  • 05:36Image Analysis
  • 06:23Results: Real-Time Monitoring of the Dynamics of Presynaptic Calcium Influx and Synaptic Vesicle Membrane Fusion in In Vitro Cultured Neurons
  • 08:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

描述了两种相关方法，以可视化突触传递所需的亚细胞事件。这些方案能够使用 体外 培养神经元的活细胞成像实时监测突触前钙流入和突触囊泡膜融合的动态。

Tags

Real-timeFluorescentSynapticAmyotrophic Lateral SclerosisSynaptic FunctionElectrophysiologyPrimary Rodent Neuronal CulturesInduced Pluripotent Stem CellsGCaMPStyryl DyeACSFPotassium ChlorideConfocal Imaging

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