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用户友好型、高通量和全自动数据采集软件，用于单颗粒冷冻电子显微镜
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
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用户友好型、高通量和全自动数据采集软件，用于单颗粒冷冻电子显微镜

DOI:
10.3791/62832-v

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07:56 min

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July 29, 2021

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, , ,
1Molecular Biophysics Unit,Indian Institute of Science, 2Gatan Inc.

Capitoli

  • 00:04Introduction
  • 00:40Data Acquisition with Latitude-S
  • 03:34Fine Alignment
  • 04:20Data Acquisition Procedure
  • 05:36Results: Automated Data Acquisition Using Latitude-S for Single-Particle Cryo-Electron Microscopy
  • 07:31Conclusion

Summary

Traduzione automatica

Traduzione automatica

单颗粒冷冻电子显微镜需要合适的软件包和用户友好的管道来实现高通量自动数据采集。在这里，我们介绍了全自动图像采集软件包Latitude-S的应用，以及用于在低剂量条件下收集玻化生物分子数据的实用管道。

Tags

1. Automated Data Acquisition Software2. Single-particle Cryo-electron Microscopy3. Structural Characterization4. Biological Macromolecules5. Pathogen Biology6. Latitude-S7. Technique Manager8. Single-Particle Automated Data Collection9. TEM Condition10. Camera Condition11. Atlas State12. Grid State13. Hole State14. Focus State15. Data State16. Defocus Values17. Microscope Settings18. Imaging Optics19. Illumination Conditions20. Camera Exposure Time

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