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Brugervenlig, høj-throughput, og fuldautomatisk dataindsamling software til single-partikel Cryo-elektron Mikroskopi
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Brugervenlig, høj-throughput, og fuldautomatisk dataindsamling software til single-partikel Cryo-elektron Mikroskopi

DOI:
10.3791/62832-v

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07:56 min

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July 29, 2021

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, , ,
1Molecular Biophysics Unit,Indian Institute of Science, 2Gatan Inc.

Capitoli

  • 00:04Introduction
  • 00:40Data Acquisition with Latitude-S
  • 03:34Fine Alignment
  • 04:20Data Acquisition Procedure
  • 05:36Results: Automated Data Acquisition Using Latitude-S for Single-Particle Cryo-Electron Microscopy
  • 07:31Conclusion

Summary

Traduzione automatica

Traduzione automatica

Enkelt-partikel cryo-elektronmikroskopi kræver en passende softwarepakke og brugervenlig rørledning til automatisk dataindsamling med høj kapacitet. Her præsenterer vi anvendelsen af en fuldautomatisk softwarepakke til billedopkøb, Latitude-S, og en praktisk pipeline til dataindsamling af vitrified biomolekyler under lavdosisforhold.

Tags

Keywords: 1. Automated Data Acquisition Software 2. Single-particle Cryo-electron Microscopy 3. Structural Characterization 4. Biological Macromolecules 5. Pathogen Biology 6. Latitude-S 7. Technique Manager 8. Single-Particle Automated Data Collection 9. TEM Condition 10. Camera Condition 11. Atlas State 12. Grid State 13. Hole State 14. Focus State 15. Data State 16. Defocus Values 17. Microscope Settings 18. Imaging Optics 19. Illumination Conditions 20. Camera Exposure Time

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