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Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
 

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Article DOI: 10.3791/62854-v • 10:07 min • October 8th, 2021
October 8th, 2021 •
1,2, 2, 3, 2
1UES Inc., 2711th Human Performance Wing, Airman Systems Directorate, Air Force Research Laboratory, Wright-Patterson AFB, 3Materials and Manufacturing Directorate, Wright-Patterson AFB

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The protocol describes the formation of robust and biocompatible DNA-laden microcapsules as multiplexed in vitro biosensors capable of tracking several ligands.

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Multifunctional Silk-based Microcapsules DNA Plasmids RNA Aptamers Riboswitches DNA-encoded Sensors Semipermeable Biocompatible Microcapsules DNA Immobilization Technique Multifunctional Biosensors In Vitro Activation Mechanisms Biological Processes Signaling Molecules Biofilms Organs Molecular Crowding Gene Activation Kinetics And Mechanism Polyethyleneimine Prime Layer Sacrificial Silica Microparticles ThermoMixer Centrifugation DNase-and RNase-free Deionized Water
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