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Fluorescensaktiverad cellsortering-radioligandbehandlad vävnad (FACS-RTT) för att bestämma det cellulära ursprunget för radioaktiv signal
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1Division of Adult Psychiatry, Department of Psychiatry,University Hospitals of Geneva, 2Department of Psychiatry,University of Geneva, 3Division of Nuclear medicine and Molecular Imaging,University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology,University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine,Imperial College London
Capitoli
- 00:04Introduction
- 00:38SPECT Camera Preparation and Calibration
- 01:47[125I]CLINDE Radiotracer Synthesis
- 04:27SPECT Acquisition
- 05:15Scan Reconstruction
- 05:57Rat Brain Extraction and Cell Isolation
- 09:46Results: FACS-RTT to Determine the Cellular Origin of Radioactive Signal
- 11:27Conclusion
Fluorescensaktiverad cellsortering-radioligandbehandlad vävnad (FACS-RTT) är ett kraftfullt verktyg för att studera rollen av 18 kDa-translokatorproteinet eller Serotonin 5HT2A-receptoruttrycket vid Alzheimers sjukdom i cellulär skala. Detta protokoll beskriver ex-vivo-appliceringen av FACS-RTT i TgF344-AD-råttmodellen.
