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Design and Development of a Model to Study the Effect of Supplemental Oxygen on the Cystic Fibrosis Airway Microbiome
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JoVE Journal Medicina
Design and Development of a Model to Study the Effect of Supplemental Oxygen on the Cystic Fibrosis Airway Microbiome

Design and Development of a Model to Study the Effect of Supplemental Oxygen on the Cystic Fibrosis Airway Microbiome

DOI:
10.3791/62888-v

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07:38 min

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August 03, 2021

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, , , , , , ,
1Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital, 2Department of Molecular Biology & Biochemistry,University of California, Irvine, 3Department of Medicine,Massachusetts General Hospital, 4Department of Microbiology & Immunology,Geisel School of Medicine at Dartmouth, 5Swiss i-research and teaching institute

Capitoli

  • 00:04Introduction
  • 00:51Artificial Sputum Medium Preparation
  • 02:28Oxygen Sparging
  • 05:25Results: Culturing in Artificial Sputum Medium
  • 07:09Conclusion

Summary

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The goal of this protocol is to develop a model system for the effect of hyperoxia on cystic fibrosis airway microbial communities. Artificial sputum medium emulates the composition of sputum, and hyperoxic culture conditions model the effects of supplemental oxygen on lung microbial communities.

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Keywords: Cystic FibrosisAirway MicrobiomeSupplemental OxygenArtificial Sputum MediumASCMASMMASBMMops BufferPHFiltrationVacuumOrbital ShakerCold RoomOxygen SpargingSerum BottleMetagenomics Sequencing

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