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研究辅助供氧对囊性纤维化气道微生物组影响的模型的设计与开发
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Design and Development of a Model to Study the Effect of Supplemental Oxygen on the Cystic Fibrosis Airway Microbiome
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研究辅助供氧对囊性纤维化气道微生物组影响的模型的设计与开发

DOI:
10.3791/62888-v

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07:38 min

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August 03, 2021

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, , , , , , ,
1Division of Pulmonary and Critical Care Medicine,Massachusetts General Hospital, 2Department of Molecular Biology & Biochemistry,University of California, Irvine, 3Department of Medicine,Massachusetts General Hospital, 4Department of Microbiology & Immunology,Geisel School of Medicine at Dartmouth, 5Swiss i-research and teaching institute

Capitoli

  • 00:04Introduction
  • 00:51Artificial Sputum Medium Preparation
  • 02:28Oxygen Sparging
  • 05:25Results: Culturing in Artificial Sputum Medium
  • 07:09Conclusion

Summary

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该协议的目标是开发一个模型系统，用于高氧对囊性纤维化气道微生物群落的影响。 人工痰培养基模拟痰液的组成，高氧培养条件模拟了辅助氧气对肺微生物群落的影响。

Tags

Cystic FibrosisAirway MicrobiomeSupplemental OxygenArtificial Sputum MediumASCMASMMASBMMops BufferPHFiltrationVacuumOrbital ShakerCold RoomOxygen SpargingSerum BottleMetagenomics Sequencing

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