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Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
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JoVE Journal Biochimica
Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

DOI:
10.3791/62904-v

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11:03 min

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September 09, 2021

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, ,
1Department of Biochemistry, School of Medicine,West Virginia University

Capitoli

  • 00:04Introduction
  • 00:56Liver Perfusion and Dissociation
  • 04:00Fatty Acid β-oxidation Assay
  • 08:59Results: The Measurement of Fatty Acid β-Oxidation in a Mouse Hepatocytes Suspension Incubated with 14C-Labeled Palmitic Acid
  • 10:24Conclusion

Summary

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Fatty acid β-oxidation is an essential metabolic pathway responsible for generating energy in many different cell types, including hepatocytes. Here, we describe a method to measure fatty acid β-oxidation in freshly isolated primary hepatocytes using 14C-labeled palmitic acid.

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Keywords: Fatty Acid Beta-oxidationMouse HepatocytesMitochondrialPeroxisomalPerfusionCollagenaseM199 Buffer

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