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离体视网膜组织样品中线粒体呼吸和糖酵解的测定
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Determination of Mitochondrial Respiration and Glycolysis in Ex Vivo Retinal Tissue Samples
DOI:

08:45 min

August 04, 2021

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Capitoli

  • 00:04Introduction
  • 01:21Hydrating Sensor Cartridge and Preparation of the Assay Medium
  • 01:55Coating Mesh Inserts of Islet Capture Microplate
  • 02:35Retinal Dissection and Retinal Punch Preparation
  • 04:21Loading the Sensor Cartridge Injection Ports and Calibration
  • 05:09Loading the Islet Capture Plate and Running Assay
  • 06:31Results: Mitochondrial Stress and Glycolytic Rate Assays
  • 07:51Conclusion

Summary

Traduzione automatica

这里描述的是使用商用生物分析仪在 离体 视网膜组织样品中进行线粒体应激测定和糖酵解速率测定的详细方案。

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