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Meccanobiologia completamente ottica Interrogazione di proteine associate al sì nel cancro umano e nelle cellule normali utilizzando un sistema multifunzionale
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1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering,University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering,University of Florida, 3Department of Electrical and Computer Engineering,University of California, 4Department of Radiation Oncology, College of Medicine,University of Florida, 5UF Health Cancer Center,University of Florida, 6Department of Physics, College of Liberal Arts and Sciences,University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine,University of Florida
Capitoli
- 00:04Introduction
- 00:53Cell Imaging
- 07:21Results: All-Optical Mechanobiology Interrogation of Yes-Associated Protein in Human Cancer and Normal Cells
- 09:11Conclusion
Questo documento presenta un protocollo graduale dettagliato su come utilizzare un sistema integrato multifunzionale e programmabile dall'utente che consente l'imaging multicanale automatico e l'analisi meccanobiologica per chiarire la meccanosensibilità della proteina associata al Sì (YAP).
Tags
Keywords: MechanobiologyYes-associated ProteinCancer CellsNormal CellsMulti-functional SystemOptical ElectrophysiologySuper-resolution ImagingLive-cell ImagingHigh-throughputSelf-drift CorrectingLong-term ImagingFluorescent MicroscopyNYCOMEnvironment ChamberMotorized StageCO2 FlowTemperature ControlGlass-bottom Petri DishConfocal ControllerMicroManagerAdhesive TapeAdhesive GlueIntelliJAMFIP
