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测量TDP-43蛋白病果蝇模型中的葡萄糖摄取

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy

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测量TDP-43蛋白病果蝇模型中的葡萄糖摄取

DOI:

10.3791/62936-v

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07:07 min

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August 03, 2021

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Suvithanandhini Loganathan*¹, Hannah E. Ball*¹, Ernesto Manzo², Daniela C. Zarnescu³

¹Department of Molecular and Cellular Biology,University of Arizona, Tucson, ²Vollum Institute,Oregon Health and Science University, ³Department of Neuroscience,University of Arizona, Tucson

Capitoli

00:04Introduction
00:41Drosophila Ventral Nerve Cord Dissections
01:43Image Acquisition
03:27Image Processing, ROI Selection, and Data Analysis
04:46Results: Glucose Uptake Measurement in Drosophila Models of TDP-43 Proteinopathy
06:32Conclusion

Summary

Traduzione automatica

受 TAR DNA 结合蛋白（TDP-43）蛋白病影响 的果蝇 运动神经元中的葡萄糖摄取增加，如基于 FRET 的遗传编码葡萄糖传感器所示。

Tags

Glucose Uptake Drosophila TDP-43 Proteinopathy FRET-based Sensor Motor Neurons ALS Models Live Imaging Central Nervous System Ventral Nerve Cord Confocal Microscopy

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