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乳腺上皮和内皮细胞球状体作为乳腺癌研究的潜在功能体外模型

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Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

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乳腺上皮和内皮细胞球状体作为乳腺癌研究的潜在功能体外模型

DOI:

10.3791/62940-v

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08:30 min

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July 12, 2021

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Giovanna Azzarito, Marta Ewa Szutkowska, Annalisa Saltari, Edwin K. Jackson, Brigitte Leeners, Marinella Rosselli, Raghvendra K. Dubey³

¹Department of Reproductive Endocrinology, Wagistrasse 14,University of Zurich and University Hospital Zurich, ²Department of Dermatology, Wagistrasse 18,University of Zurich and University Hospital Zurich, ³Department of Pharmacology and Chemical Biology,University of Pittsburgh

Capitoli

00:04Introduction
00:49Spheroid Formation
03:55Spheroid Immunohistochemistry Study
05:24Live/Dead Staining in Spheroids
05:56Results: Generation of Mammary Epithelial and Endothelial Cell Spheroids
07:41Conclusion

Summary

Traduzione automatica

乳腺上皮细胞和内皮细胞之间的串扰对乳腺癌进展、肿瘤生长和转移有重要贡献。在这项研究中，球状体是由乳腺癌细胞与血管和/或淋巴内皮细胞一起制成的，并证明了它们作为乳腺癌研究 的体外 系统的适用性。

Tags

Keywords: Breast Cancer Cell Spheroids 3D Model Mammary Epithelial Cells Endothelial Cells Cancer Cell-endothelial Cell Interactions Proliferation Metastasis In Vitro Model Cell Distribution Dye Staining Enzymatic Detachment Cell Counting Cell Suspension Cell Co-culture

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