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Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion
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JoVE Journal Medicina
Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion

Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion

DOI:
10.3791/62942-v

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06:27 min

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February 01, 2022

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, , , ,
1Laboratory of Pharmaceutical Pharmacology,Latvian Institute of Organic Synthesis

Capitoli

  • 00:04Introduction
  • 00:41Heart Staining and Slicing
  • 02:00Brain Staining and Slicing
  • 03:16Macro Photography
  • 04:26Results: Rodent Tissue: Slicing, Staining and Digital Macro Photography
  • 05:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

Presented here is a protocol for the standardized methodology of rodent tissue preparation after the ischemia-reperfusion experiment and guidelines for establishing lighting and camera setups for high-resolution image acquisition. This method is applicable to all experimental small-animal organ photography.

Tags

RodentHeartBrainPreparationDigitalMacroPhotographyIschemiaReperfusionStainingLightingCamera SetupPhotography TechniquesImage AcquisitionPlanimetric AnalysisMacrophotographyMethylene BluePerfusionTriphenyl Tetrazolium ChloridePBSBrain MatrixCoronal Slices

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