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Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization
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Neuroscienze
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JoVE Journal Neuroscienze
Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

DOI:
10.3791/62945-v

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08:16 min

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September 20, 2021

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,
1Center for Hypothalamic Research and Department of Internal Medicine,UTSouthwestern Medical Center at Dallas

Capitoli

  • 00:04Introduction
  • 00:39The Mouse Jugular-nodose Ganglia Sample Collection
  • 02:51Pretreatment of the Slides and In Situ Hybridization (ISH)
  • 04:51Microscopy and Data Analysis
  • 05:34Results: Microscopic Analysis of the Mouse Vagal Afferent Neurons After Multiplex in Situ Hybridization
  • 07:34Conclusion

Summary

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Multiplex in situ hybridization (ISH) was employed to simultaneously visualize the transcripts for two G protein-coupled receptors and one transcription factor in the entire vagal ganglionic complex of the adult mouse. This protocol could be used to generate accurate maps of the transcriptional profiles of vagal afferent neurons.

Tags

G Protein-coupled ReceptorVagal Afferent NeuronsMultiplex In Situ HybridizationMouseTranscriptional ProfilingCryosectioningHistological Techniques

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