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培养的星形胶质细胞中线粒体系统的实时成像
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Live-imaging of Mitochondrial System in Cultured Astrocytes
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培养的星形胶质细胞中线粒体系统的实时成像

DOI:
10.3791/62957-v

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06:20 min

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November 16, 2021

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, , ,
1Department of Psychiatry, Center for Psychiatric Neurosciences,Lausanne University Hospital (CHUV) and University of Lausanne

Capitoli

  • 00:04Introduction
  • 00:44Rat Hippocampal Astrocyte Primary Culture
  • 01:25Long-Term Monitoring of the Mitochondrial System
  • 02:13Analysis of Individual Mitochondrial Morphology and LV-G1-MitoTimer Ratio
  • 03:54Analysis of Mitochondrial Motility
  • 04:28Data Transformation, Normalization, and Statistical Analysis
  • 04:51Results: Analysis of the Effects of H2O2 on the Mitochondrial System of Astrocytes
  • 05:53Conclusion

Summary

Traduzione automatica

Traduzione automatica

本文介绍了配备MitoTimer生物传感器的星形胶质细胞培养物的线粒体延时成像方法，以及由此产生的线粒体动力学，迁移率，形态，生物发生，氧化还原状态和周转的多参数分析。

Tags

Keywords: Live-imagingMitochondrial SystemCultured AstrocytesMicroscopyMitochondrial DynamicsLentiviral VectorMitoTimerFluorescence ImagingImage ProcessingSegmentationMitochondrial Measurements

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